ResA

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  • Description: extracytoplasmic thioredoxin, cytochrome c biogenesis, reduces disulfide bonds in apo-cytochrome prior to the attachment of heme

Gene name resA
Synonyms ypxA
Essential no
Product extracytoplasmic thioredoxin
Function cytochrome c biogenesis
Gene expression levels in SubtiExpress: resA
MW, pI 20 kDa, 8.37
Gene length, protein length 543 bp, 181 aa
Immediate neighbours resB, rluB
Sequences Protein DNA DNA_with_flanks
Genetic context
ResA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ResA expression.png
























Categories containing this gene/protein

respiration

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, ResD regulon

The gene

Basic information

  • Locus tag: BSU23150

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: ResA subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (ResD) PubMed
    • expressed under conditions of phosphate limitation (PhoP) PubMed
    • repressed by glucose (CcpA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Christopher T C Hodson, Allison Lewin, Lars Hederstedt, Nick E Le Brun
The active-site cysteinyls and hydrophobic cavity residues of ResA are important for cytochrome c maturation in Bacillus subtilis.
J Bacteriol: 2008, 190(13);4697-705
[PubMed:18456809] [WorldCat.org] [DOI] (I p)

Allison Lewin, Allister Crow, Christopher T C Hodson, Lars Hederstedt, Nick E Le Brun
Effects of substitutions in the CXXC active-site motif of the extracytoplasmic thioredoxin ResA.
Biochem J: 2008, 414(1);81-91
[PubMed:18422485] [WorldCat.org] [DOI] (I p)

Allison Lewin, Allister Crow, Arthur Oubrie, Nick E Le Brun
Molecular basis for specificity of the extracytoplasmic thioredoxin ResA.
J Biol Chem: 2006, 281(46);35467-77
[PubMed:16971393] [WorldCat.org] [DOI] (P p)

Soo-Keun Choi, Milton H Saier
Mechanism of CcpA-mediated glucose repression of the resABCDE operon of Bacillus subtilis.
J Mol Microbiol Biotechnol: 2006, 11(1-2);104-10
[PubMed:16825793] [WorldCat.org] [DOI] (P p)

M M Nakano, Y Zhu
Involvement of ResE phosphatase activity in down-regulation of ResD-controlled genes in Bacillus subtilis during aerobic growth.
J Bacteriol: 2001, 183(6);1938-44
[PubMed:11222591] [WorldCat.org] [DOI] (P p)

S M Birkey, W Liu, X Zhang, M F Duggan, F M Hulett
Pho signal transduction network reveals direct transcriptional regulation of one two-component system by another two-component regulator: Bacillus subtilis PhoP directly regulates production of ResD.
Mol Microbiol: 1998, 30(5);943-53
[PubMed:9988472] [WorldCat.org] [DOI] (P p)

G Sun, E Sharkova, R Chesnut, S Birkey, M F Duggan, A Sorokin, P Pujic, S D Ehrlich, F M Hulett
Regulators of aerobic and anaerobic respiration in Bacillus subtilis.
J Bacteriol: 1996, 178(5);1374-85
[PubMed:8631715] [WorldCat.org] [DOI] (P p)

  • Additional publications

PubMed