remA

168

transcriptional regulator of the extracellular matrix genes, acts in parallel to [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR], [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB], and [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]

locus: BSU_15670

Molecular weight: 9.71 kDa

pI: 7.18

Protein length: 89 aa

Gene length: 270 bp

function: control of biofilm formation

product: regulator of the extracellular matrix genes

essential: no

ec: null

synonyms: remA, ylzA

List of homologs in different organisms, belongs to COG2052 (Galperin et al., 2021)

Gene

Coordinates: 1,641,672  1,641,941

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The protein

Catalyzed reaction/ biological activity

• binds [gene|FB2220730013A98D7C979062A4A451518B92DF09|opuAA], [gene|8C13E1EF5437DD8F9906643DEDCBB18DABE3B9E3|tapA], and [gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA] promoter regions (upstream of the actual promoter) to activate transcription [Pubmed|23646920]
• binds DNA with high co-operativity due to low conserrvation of binding sites [Pubmed|23646920]

Protein family

• RemA family (single member, according to UniProt)

Structure

• [PDB|7BM2] [pubmed|34588455]
• [AF|Q7WY72]

Biological materials

Mutant

• BKE15670 ([gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTACGTTCCCCCTG,  downstream forward: _UP4_GATGAAGGGCAGGGGTAATT
• BKK15670 ([gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTACGTTCCCCCTG,  downstream forward: _UP4_GATGAAGGGCAGGGGTAATT

labs

• [wiki|Daniel Kearns], Indiana University, Bloomington, USA, [http://www.bio.indiana.edu/facultyresearch/faculty/Kearns.html homepage]

References

Reviews

• 24988880,35289951

Original Publications

• 19363116,22329926,23123912,23396918,23646920,25035996,34588455