recQ

recQ
168

ATP-dependent DNA helicase, acts together with [protein|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ] in replication fork maintenance

locus
BSU_19220
Molecular weight
67.18 kDa
pI
5.92
Protein length
Gene length
function
replication fork maintenance
product
ATP-dependent DNA helicase
essential
no
synonyms
yocI

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0514 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,094,010 → 2,095,785
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The protein
Catalyzed reaction/ biological activity
unwinds covalently closed ds DNA (in complex with [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SSB]) [pubmed|10360177]
[protein|search|RecQ ]controls [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|Topo III] (de)catenation activity [pubmed|11497238,10360177]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
[wiki|helicase family] (according to UniProt)
[wiki|Domains]
[wiki|Helicase ATP-binding domain] (aa 27-196) (according to UniProt)
[wiki|Helicase C-terminal domain] (aa 216-367) (according to UniProt)
HRDC domain (aa 513-591) (according to UniProt)
Structure
[PDB|4Q47] ([protein|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|recQ] from Deinococcus radiodurans, 40% identity) [pubmed|25243132]
[AF|O34748]
Paralogous protein(s)
[protein|4A4FCD876C3CA1D821792803CA2A0CB2E97D3F5E|recS]
[wiki|Localization]
associated with the [wiki|replisome] [pubmed|17853894]
Expression and Regulation
Operons
genes
[gene|E85341621B3835F63F07C506E08B73A92E588B1F|bsrB]-[gene|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|recQ]-[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]
description
[Pubmed|22383849]
Open in new tab

[gene|E85341621B3835F63F07C506E08B73A92E588B1F|bsrB]→[gene|BEBA71A2EF56DAEAF80924856DA68124805BB158|yocH]

2024-03-11 20:30:25

Jstuelk

70

c46135c8ecda918f2cefaaabf9488391edf3d4e8

9354DC65CD7CD9CA6CB2929005BDACFC092A2FE9

Biological materials
Mutant
GP3543 (Δ[gene|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|recQ]::kan), available in [wiki|Jörg Stülke]'s lab
MGNA-B414 (yocI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1413 NBRP B. subtilis, Japan]
BKE19220 (Δ[gene|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|recQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACGTGTAAACCTCGCT,  downstream forward: _UP4_TAAAAAAACAGAAAAACAAT
BKK19220 (Δ[gene|E6EA8098E37A4A2F7370920AC1A500EE4319AAA2|recQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACGTGTAAACCTCGCT,  downstream forward: _UP4_TAAAAAAACAGAAAAACAAT
References
Reviews
11497238,22933559,15217989,32286623
Original Publications
25246477,25243132,31675066,17853894

E6EA8098E37A4A2F7370920AC1A500EE4319AAA2

Page visits: 2748

Time of last update: 2024-03-28 12:35:09

Author of last update: MBenda