RecJ

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  • Description: single-strand DNA-specific exonuclease

Gene name yrvE
Synonyms recJ
Essential no
Product single-strand DNA-specific exonuclease
Function unknown
Gene expression levels in SubtiExpress: yrvE
Interactions involving this protein in SubtInteract: YrvE
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation)
MW, pI 87 kDa, 5.181
Gene length, protein length 2358 bp, 786 aa
Immediate neighbours apt, yrvD
Sequences Protein DNA DNA_with_flanks
Genetic context
YrvE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YrvE expression.png















Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

stringent response, Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU27620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: recJ family (according to Swiss-Prot)
  • Paralogous protein(s): YorK

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

  • Mutant:
    • GP895 (recJ::kan), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol Genet Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

V A Sutera, E S Han, L A Rajman, S T Lovett
Mutational analysis of the RecJ exonuclease of Escherichia coli: identification of phosphoesterase motifs.
J Bacteriol: 1999, 181(19);6098-102
[PubMed:10498723] [WorldCat.org] [DOI] (P p)