Difference between revisions of "RecJ"

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m (Jstuelk moved page YrvE to RecJ: 26001966)
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|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yrvE''
+
|''recJ''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''recJ ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yrvE''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
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|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair
 
|style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27620 yrvE]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27620 recJ]
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=YrvE YrvE]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=YrvE RecJ]
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=yrvE yrvE]'''
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=yrvE recJ]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 87 kDa, 5.181   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 87 kDa, 5.181   
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
** [[SsbA]]-[[YrvE]] {{PubMed|21170359}}
+
** [[SsbA]]-[[RecJ]] {{PubMed|21170359}}
  
 
* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
 
* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[yrvE]]-[[apt]]'' {{PubMed|15241682}}
+
* '''Operon:''' ''[[recJ]]-[[apt]]'' {{PubMed|15241682}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recJ_2823419_2825779_-1 yrvE] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recJ_2823419_2825779_-1 yrvE] {{PubMed|22383849}}
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* '''Mutant:'''
 
* '''Mutant:'''
** GP895 (''recJ''::''kan''), available in [[Stülke]] lab  
+
** GP895 (''[[recJ]]''::''kan''), available in [[Jörg Stülke]]'s lab  
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 11:06, 7 July 2015

  • Description: single-strand DNA-specific exonuclease

Gene name recJ
Synonyms yrvE
Essential no
Product single-strand DNA-specific exonuclease
Function DNA repair
Gene expression levels in SubtiExpress: recJ
Interactions involving this protein in SubtInteract: RecJ
Metabolic function and regulation of this protein in SubtiPathways:
recJ
MW, pI 87 kDa, 5.181
Gene length, protein length 2358 bp, 786 aa
Immediate neighbours apt, yrvD
Sequences Protein DNA DNA_with_flanks
Genetic context
YrvE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YrvE expression.png















Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

stringent response, Efp-dependent proteins

The gene

Basic information

  • Locus tag: BSU27620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: recJ family (according to Swiss-Prot)
  • Paralogous protein(s): YorK

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Hanjing Yang, Cameron Sikavi, Katherine Tran, Shauna M McGillivray, Victor Nizet, Madeline Yung, Aileen Chang, Jeffrey H Miller
Papillation in Bacillus anthracis colonies: a tool for finding new mutators.
Mol Microbiol: 2011, 79(5);1276-93
[PubMed:21205011] [WorldCat.org] [DOI] (I p)

Audrey Costes, François Lecointe, Stephen McGovern, Sophie Quevillon-Cheruel, Patrice Polard
The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.
PLoS Genet: 2010, 6(12);e1001238
[PubMed:21170359] [WorldCat.org] [DOI] (I e)

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol Genet Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

V A Sutera, E S Han, L A Rajman, S T Lovett
Mutational analysis of the RecJ exonuclease of Escherichia coli: identification of phosphoesterase motifs.
J Bacteriol: 1999, 181(19);6098-102
[PubMed:10498723] [WorldCat.org] [DOI] (P p)