Difference between revisions of "RecA"

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* Gene name: recA
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* '''Description:''' write here <br/><br/>
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* Gene product: RecA
 
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* Function: multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)
 
  
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This gene is involved in the repair of DNA after activation of the stress response system.
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{| align="right" border="1" cellpadding="2"
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|-
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''recA''
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|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''recE ''
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|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' ||
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || multifunctional SOS repair regulator
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || involved in DNA repair and homologous recombination
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|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 37 kDa, 4.883 
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|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1041 bp, 347 aa
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||
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|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/recA_nucleotide.txt    Gene sequence      (+200bp)  ]'''
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/recA_protein.txt Protein sequence]'''
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|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:recA_context.gif]]
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|-
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|}
  
 
__TOC__
 
__TOC__
  
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=Gene=
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<br/><br/>
 +
 
 +
=The gene=
 +
 
 +
=== Basic information ===
 +
 
 +
* '''Coordinates:'''
 +
 
 +
===Phenotypes of a mutant ===
 +
 
 +
=== Database entries ===
 +
 
 +
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/recA.html]
 +
 
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10721]
 +
 
 +
=== Additional information===
 +
 
 +
 
 +
=The protein=
 +
 
 +
=== Basic information/ Evolution ===
 +
 
 +
* '''Catalyzed reaction/ biological activity:'''
 +
 
 +
* '''Protein family:'''
 +
 
 +
* '''Paralogous protein(s):'''
 +
 
 +
=== Extended information on the protein ===
 +
 
 +
* '''Kinetic information:'''
 +
 
 +
* '''Domains:'''
 +
 
 +
* '''Modification:'''
 +
 
 +
* '''Cofactor(s):'''
 +
 
 +
* '''Effectors of protein activity:'''
 +
 
 +
* '''Interactions:'''
 +
 
 +
* '''Localization:'''
 +
 
 +
=== Database entries ===
 +
 
 +
* '''Structure:'''
 +
 
 +
* '''Swiss prot entry:'''
 +
 
 +
* '''KEGG entry:'''
 +
 
 +
* '''E.C. number:'''
 +
 
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=== Additional information===
 +
 
 +
=Expression and regulation=
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* '''Operon:'''
  
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{| class="wikitable" border="1"
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* '''Sigma factor:'''
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|-
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| '''Operon'''
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* '''Regulation:'''
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| recABC
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|-
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* '''Regulatory mechanism:'''
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| '''Sigma Factor'''
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−
|
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* '''Additional information:'''
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|}
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 +
=Biological materials =
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* '''Mutant:'''
 +
 
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* '''Expression vector:'''
 +
       
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* '''lacZ fusion:'''
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* '''GFP fusion:'''
  
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=Protein=
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* '''two-hybrid system:'''  
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''Please add in this section information about localization, the catalyzed reaction (in case of enzymes), domains, interactions, and effectors of its activity...''. We can write something here...
 
  
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* '''Localization''': Membrane (Au et al, 2005)
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* '''Antibody:'''
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This protein is known to localize in the membrane in the exponential phase, but not in the stationary phase, where the distribution is spotty.
 
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* '''Interaction''': Interacts with [[LexA]]
 
  
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=Biological Material=
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=Labs working on this gene/protein=
  
 +
=Your additional remarks=
  
 
=References=
 
=References=
  
−
Au et al. 2005, J. Bact. 187:7655-7666 [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290]
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 08:42, 30 January 2009

  • Description: write here

Gene name recA
Synonyms recE
Essential
Product multifunctional SOS repair regulator
Function involved in DNA repair and homologous recombination
MW, pI 37 kDa, 4.883
Gene length, protein length 1041 bp, 347 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
RecA context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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