Difference between revisions of "RbgA"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16050 rbgA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16050 rbgA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RbgA RbgA]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 9.528   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 9.528   
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Binds and hydrolyzes GTP and readily exchanges GDP for GTP
+
* '''Catalyzed reaction/ biological activity:'''  
 +
** proper positioning of [[rplF|L6]] in the large subunit of the [[ribosome]] prior to incorporation of [[rplP|L16]] {{PubMed|25330043}}
 +
** Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  
 
* '''Protein family:''' MTG1 subfamily (according to Swiss-Prot) Era/Obg family
 
* '''Protein family:''' MTG1 subfamily (according to Swiss-Prot) Era/Obg family
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* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
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** [[RbgA]]-[[RplF]] {{PubMed|25330043}}
  
 
* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
 
* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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==Original publications==
 
==Original publications==
<pubmed>12427945, 16390447 16997968 23325847 22267738 23700310 24335279</pubmed>
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<pubmed>12427945, 16390447 16997968 23325847 22267738 23700310 24335279 25330043</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:02, 27 October 2014

  • Description: assembly of the 50S subunit of the ribosome

Gene name rbgA
Synonyms ylqF
Essential yes PubMed
Product GTPase
Function ribosome assembly
Gene expression levels in SubtiExpress: rbgA
Interactions involving this protein in SubtInteract: RbgA
MW, pI 31 kDa, 9.528
Gene length, protein length 846 bp, 282 aa
Immediate neighbours rplS, rnhB
Sequences Protein DNA DNA_with_flanks
Genetic context
YlqF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RbgA expression.png















Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16050

Phenotypes of a mutant

  • essential PubMed
  • depletion confers a slow growth phenotype, and induces an abnormality of the in vivo ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes PubMed
  • premature 45S particles accumulated in RbgA-depleted cells lack several proteins, including L16, L27, L28, L33, L35 and L36 PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • proper positioning of L6 in the large subunit of the ribosome prior to incorporation of L16 PubMed
    • Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: MTG1 subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
    • RNA-binding domain at the C-terminus PubMed
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 94 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 568 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)


Original publications