radC

168

probable DNA repair protein

locus: BSU_28040

Molecular weight: 26.00 kDa

pI: 7.83

Protein length: 231 aa

Gene length: 696 bp

function: DNA repair

product: probable DNA repair protein

essential: no

synonyms: radC, ysxA

List of homologs in different organisms, belongs to COG2003 (Galperin et al., 2021)

Gene

Coordinates: 2,861,840  2,862,535

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The protein

Protein family

• UPF0758 family (single member, according to UniProt)

[wiki|Domains]

• MPN domain (aa 109-231) (according to UniProt)

Structure

• [PDB|2QLC] (C-terminal domain of Chlorobium tepidum RadC, 46% identity)
• [AF|Q02170]

Modification

• phosphorylated on Arg-59 [Pubmed|22517742]

[wiki|Localization]

• cytoplasm (Homogeneous) [Pubmed|16479537]

Biological materials

Mutant

• GP3561 (Δ[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]::aphA3), available in [wiki|Jörg Stülke]'s lab
• MGNA-B011 (ysxA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1010 NBRP B. subtilis, Japan]
• BKE28040 ([gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCACTCCTTTTCTTTCC,  downstream forward: _UP4_TAACACTTTTTTTTTCGTCG
• BKK28040 ([gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCACTCCTTTTCTTTCC,  downstream forward: _UP4_TAACACTTTTTTTTTCGTCG

References

• 17434969,11948146,11918817,16479537,12775685,18179421,22517742,24009114,21926231,22383849,26091431