RacE

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  • Description: glutamate racemase

Gene name racE
Synonyms glr
Essential yes PubMed
Product glutamate racemase
Function peptidoglycan precursor biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Cell wall
MW, pI 29 kDa, 4.858
Gene length, protein length 816 bp, 272 aa
Immediate neighbours gerM, ysmB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RacE context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU28390

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-glutamate = D-glutamate (according to Swiss-Prot)
  • Protein family: aspartate/glutamate racemases family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1ZUW (with D-glutamate)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

M Ashley Spies, Joseph G Reese, Dylan Dodd, Katherine L Pankow, Steven R Blanke, Jerome Baudry
Determinants of catalytic power and ligand binding in glutamate racemase.
J Am Chem Soc: 2009, 131(14);5274-84
[PubMed:19309142] [WorldCat.org] [DOI] (I p)

Eduard Puig, Edgar Mixcoha, Mireia Garcia-Viloca, Angels González-Lafont, José M Lluch
How the substrate D-glutamate drives the catalytic action of Bacillus subtilis glutamate racemase.
J Am Chem Soc: 2009, 131(10);3509-21
[PubMed:19227983] [WorldCat.org] [DOI] (I p)

Sergey N Ruzheinikov, Makie A Taal, Svetlana E Sedelnikova, Patrick J Baker, David W Rice
Substrate-induced conformational changes in Bacillus subtilis glutamate racemase and their implications for drug discovery.
Structure: 2005, 13(11);1707-13
[PubMed:16271894] [WorldCat.org] [DOI] (P p)

Keitarou Kimura, Lam-Son Phan Tran, Yoshifumi Itoh
Roles and regulation of the glutamate racemase isogenes, racE and yrpC, in Bacillus subtilis.
Microbiology (Reading): 2004, 150(Pt 9);2911-2920
[PubMed:15347750] [WorldCat.org] [DOI] (P p)