Difference between revisions of "QdoI"

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* '''Description:''' Fe-containing quercetin 2,3-dioxygenase <br/><br/>
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=[http://uxegyjyga.co.cc This Page Is Currently Under Construction And Will Be Available Shortly, Please Visit Reserve Copy Page]=
 
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=[http://uxegyjyga.co.cc CLICK HERE]=
 
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* '''Description:''' Fe-containing quercetin 2,3-dioxygenase &lt;br/>&lt;br/>
 
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxaH]]'', ''[[yxaF]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yxaH]]'', ''[[yxaF]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&amp;#91;EMBLCDS:CAB16035&amp;#93;+-newId sequences] &lt;br/> (Barbe ''et al.'', 2009)'''
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB16035&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
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|colspan="2" | '''Genetic context''' &lt;br/> [[Image:yxaG_context.gif]]
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|colspan="2" | '''Genetic context''' <br/> [[Image:yxaG_context.gif]]
  &lt;div align="right"> &lt;small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]&lt;/small>&lt;/div>
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  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
 
|}
 
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__TOC__
 
__TOC__
  
&lt;br/>&lt;br/>
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<br/><br/>
  
 
=The gene=
 
=The gene=
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Quercetin + O&lt;sub>2&lt;/sub> = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H&lt;sup>+&lt;/sup> (according to Swiss-Prot)  
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* '''Catalyzed reaction/ biological activity:''' Quercetin + O<sub>2</sub> = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H<sup>+</sup> (according to Swiss-Prot)  
  
 
* '''Protein family:'''bicupin family
 
* '''Protein family:'''bicupin family
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=References=
 
=References=
  
&lt;pubmed>17483215,14741339,,17483215,15317768,10746760, 20460727,14697267,, &lt;/pubmed>
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<pubmed>17483215,14741339,,17483215,15317768,10746760, 20460727,14697267,, </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:39, 24 November 2010

  • Description: Fe-containing quercetin 2,3-dioxygenase

Gene name qdoI
Synonyms yxaG
Essential no
Product Fe-containing quercetin 2,3-dioxygenase
Function resistance to plant product quercetin
MW, pI 37,4 kDa, 5.562
Gene length, protein length 1011 bp, 337 aa
Immediate neighbours yxaH, yxaF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YxaG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU39980

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+ (according to Swiss-Prot)
  • Protein family:bicupin family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): Fe(2+)
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kazutake Hirooka, Yasutaro Fujita
Excess production of Bacillus subtilis quercetin 2,3-dioxygenase affects cell viability in the presence of quercetin.
Biosci Biotechnol Biochem: 2010, 74(5);1030-8
[PubMed:20460727] [WorldCat.org] [DOI] (I p)

Kazutake Hirooka, Satoshi Kunikane, Hiroshi Matsuoka, Ken-Ichi Yoshida, Kanako Kumamoto, Shigeo Tojo, Yasutaro Fujita
Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids.
J Bacteriol: 2007, 189(14);5170-82
[PubMed:17483215] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Yo-Hei Ohki, Makiko Murata, Masaki Kinehara, Hiroshi Matsuoka, Takenori Satomura, Reiko Ohki, Miyuki Kumano, Kunio Yamane, Yasutaro Fujita
Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons: identification of its binding site and functional analysis of lmrB and yxaGH.
J Bacteriol: 2004, 186(17);5640-8
[PubMed:15317768] [WorldCat.org] [DOI] (P p)

Laura Bowater, Shirley A Fairhurst, Victoria J Just, Stephen Bornemann
Bacillus subtilis YxaG is a novel Fe-containing quercetin 2,3-dioxygenase.
FEBS Lett: 2004, 557(1-3);45-8
[PubMed:14741339] [WorldCat.org] [DOI] (P p)

Jim M Dunwell, Alan Purvis, Sawsan Khuri
Cupins: the most functionally diverse protein superfamily?
Phytochemistry: 2004, 65(1);7-17
[PubMed:14697267] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)