qdoI

168

Fe-containing quercetin 2,3-dioxygenase

locus: BSU_39980

Molecular weight: 37.43 kDa

pI: 5.56

Protein length: 337 aa

Gene length: 1014 bp

function: resistance to plant product quercetin

product: Fe-containing quercetin 2,3-dioxygenase

essential: no

synonyms: qdoI, yxaG

List of homologs in different organisms, belongs to COG1917 (Galperin et al., 2021)

Gene

Coordinates: 4,106,245  4,107,258

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The protein

Catalyzed reaction/ biological activity

• oxidation of the flavonol quercetin with dioxygen, cleaving the central heterocyclic ring and releasing CO
• O₂ + quercetin --> 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO (according to UniProt)

Protein family

• bicupin family

[wiki|Domains]

• 2 [wiki|cupin 2 domain]s (aa 55 ... 110, aa 226 ... 281)

[wiki|Cofactors]

• Mn(II) (preferred), but also active with Fe(II) snd Co(II) [Pubmed|22084064]

Structure

• [PDB|1Y3T] [Pubmed|15628860]
• [PDB|2H0V]
• [AF|P42106]

Biological materials

Mutant

• MGNA-B684 (yxaG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1683 NBRP B. subtilis, Japan]
• BKE39980 ([gene|6A68EF7BF16FC2F2C7E068F52901EF2BFD6EBF8A|qdoI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTATCGCTTCCTCCAA,  downstream forward: _UP4_TAAAGATGACAAGATTGTAA
• BKK39980 ([gene|6A68EF7BF16FC2F2C7E068F52901EF2BFD6EBF8A|qdoI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTATCGCTTCCTCCAA,  downstream forward: _UP4_TAAAGATGACAAGATTGTAA

References

Reviews

• 9056848,10730195

Original Publications

• 17483215,14741339,15039076,15317768,10746760,20460727,14697267,22084064,16411777,27170159,15628860