pyrR

168

transcriptional termination factor of the pyr operon

locus: BSU_15470

Molecular weight: 20.12 kDa

pI: 4.99

Protein length: 181 aa

Gene length: 546 bp

function: regulation of pyrimidine biosynthesis

product: transcriptional termination protein with minor uracil phosphoribosyltransferase activity

essential: no

ec: 2.4.2.9

synonyms: pyrR

List of homologs in different organisms, belongs to COG2065 (Galperin et al., 2021)

Gene

Coordinates: 1,618,304  1,618,849

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The protein

Catalyzed reaction/ biological activity

• diphosphate + UMP --> 5-phospho-α-D-ribose 1-diphosphate + uracil (according to UniProt)

Protein family

• [wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)

Structure

• [PDB|1A3C] (dimeric form)
• [AF|P39765]

Effectors of protein activity

• UMP and UTP stimulate the RNA-binding activity of PyrR, resulting of transcription termination of the [gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]-[gene|A5C26E10085868A0A193C450DF58D54C636B7C2D|pyrP]-[gene|B83462A50FDDD20FE9BD4681824E30AAD10F506B|pyrB]-[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]-[gene|E7741ED6F15044900BDC5F0584246469D2D4D586|pyrAA]-[gene|D57DC8A53E52BC4A2EA894E3C9FD983592882918|pyrAB]-[gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]-[gene|E533BEF28579CD04B24E2C728737269E35224450|pyrD]-[gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]-[gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE] operon [pubmed|31100987]

Biological materials

Mutant

• BKE15470 ([gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTGACACCTCACAG,  downstream forward: _UP4_GCCATTTATGAAAACGAATA
• BKK15470 ([gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTGACACCTCACAG,  downstream forward: _UP4_GCCATTTATGAAAACGAATA

References

Reviews

• 28031352,31100987,32850966

Original Publications

• 8935652,8206849,15716449,11726695,9488732,9551555,12896995,9932459,8955403,8824632,12482852,1709162,7798145,11948166,8759868,7868607,9488732,17322189