PyrR

• Description: transcriptional antiterminator of the pyr operon
  
  

Gene name	pyrR
Synonyms
Essential	no
Product	transcriptional antiterminator with minor uracil phosphoribosyltransferase activity
Function	regulation of pyrimidine biosynthesis
Gene expression levels in SubtiExpress: pyrR
Metabolic function and regulation of this protein in SubtiPathways: pyrR
MW, pI	20 kDa, 4.991
Gene length, protein length	543 bp, 181 aa
Immediate neighbours	ylyB, pyrP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, transcription factors and their control, RNA binding regulators

This gene is a member of the following regulons

PyrR regulon

The gene

Basic information

• Locus tag: BSU15470

Database entries

• BsubCyc: BSU15470

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: PyrR subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PyrR-PyrR PubMed

• Localization:

Database entries

• BsubCyc: BSU15470

• Structure: 1A3C (dimeric form)

• UniProt: P39765

• KEGG entry: [3]

• E.C. number: 2.4.2.9

Expression and regulation

• Operon: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE PubMed

• Expression browser: pyrR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the absence of uridine nucleotides (PyrR) PubMed

• Regulatory mechanism:
  • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 689 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2257 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: