pyrK

168

dihydroorotic acid dehydrogenase (electron transfer subunit)

locus: BSU_15530

Molecular weight: 27.95 kDa

pI: 5.58

Protein length: 256 aa

Gene length: 771 bp

function: pyrimidine biosynthesis

product: dihydroorotic acid dehydrogenase (electron transfer subunit)

essential: no

synonyms: pyrK, pyrDII, ylxD

List of homologs in different organisms, belongs to COG0543 (Galperin et al., 2021)

Gene

Coordinates: 1,626,948 1,627,718

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The protein

Protein family

• PyrK family (single member, according to UniProt)

[wiki|Domains]

• [wiki|FAD-binding FR-type domain] (aa 1-101) (according to UniProt)

[wiki|Cofactors]

• [2Fe-2S] cluster [pubmed|29292548,11188687]
• FAD [pubmed|11188687]

Structure

• [PDB|1EP1] (from Lactococcus lactis, 46% identity) [pubmed|11188687]
• [AF|P25983]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• BKE15530 ([gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACATACTGTCAAATACGCTT, downstream forward: _UP4_AAAGCTCAGGAGGTGGCGCT
• BKK15530 ([gene|C20A05AE4CE8AEE0DA767334935CC64106A7FCCB|pyrK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACATACTGTCAAATACGCTT, downstream forward: _UP4_AAAGCTCAGGAGGTGGCGCT

References

• 8206849,8759868,1709162,10545205,15378759,11188687