PyrH

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  • Description: uridylate kinase

Gene name pyrH
Synonyms smbA
Essential no
Product uridylate kinase
Function pyrimidine biosynthesis
Gene expression levels in SubtiExpress: pyrH
Metabolic function and regulation of this protein in SubtiPathways:
Pyrimidines, Nucleotides (regulation)
MW, pI 25 kDa, 5.101
Gene length, protein length 720 bp, 240 aa
Immediate neighbours tsf, frr
Sequences Protein DNA DNA_with_flanks
Genetic context
PyrH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PyrH expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU16510

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + UMP = ADP + UDP (according to Swiss-Prot)
  • Protein family: UMP kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2JJX (from Bacillus anthracis, 42% identity, 64% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Cristina Gagyi, Nadia Bucurenci, Ovidiu Sîrbu, Gilles Labesse, Mihaela Ionescu, Augustin Ofiteru, Liliane Assairi, Stéphanie Landais, Antoine Danchin, Octavian Bârzu, Anne-Marie Gilles
UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity.
Eur J Biochem: 2003, 270(15);3196-204
[PubMed:12869195] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)