Difference between revisions of "PyrF"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[pyrR]]-[[pyrP]]-[[pyrB]]-[[pyrC]]-[[pyrAA]]-[[pyrAB]]-[[pyrK]]-[[pyrD]]-[[pyrF]]-[[pyrE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8206849 PubMed] |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1709162 PubMed] |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** induced in the absence of uridine nucleotides ([[PyrR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8206849 PubMed] | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[PyrR]]: [[RNA switch]], transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) [http://www.ncbi.nlm.nih.gov/sites/entrez/8955403 PubMed] | ||
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 21:10, 8 June 2009
- Description: orotidine 5'-phosphate decarboxylase
| Gene name | pyrF |
| Synonyms | |
| Essential | no |
| Product | orotidine-5'-phosphate decarboxylase |
| Function | pyrimidine biosynthesis |
| MW, pI | 25 kDa, 5.835 |
| Gene length, protein length | 717 bp, 239 aa |
| Immediate neighbours | pyrD, pyrE |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU15550
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Orotidine 5'-phosphate = UMP + CO2 (according to Swiss-Prot)
- Protein family: Type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1DBT
- Swiss prot entry: P25971
- KEGG entry: [3]
- E.C. number: 4.1.1.23
Additional information
Expression and regulation
- Regulatory mechanism:
- PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
