pyrF

168

orotidine 5-phosphate decarboxylase

locus: BSU_15550

Molecular weight: 25.84 kDa

pI: 5.84

Protein length: 239 aa

Gene length: 720 bp

function: pyrimidine biosynthesis

product: orotidine-5-phosphate decarboxylase

essential: no

ec: 4.1.1.23

synonyms: pyrF

List of homologs in different organisms, belongs to COG0284 (Galperin et al., 2021)

Gene

Coordinates: 1,628,622  1,629,341

Phenotypes of a mutant

• poor growth [pubmed|28189581]
• non-transformable [pubmed|28189581]

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The protein

Catalyzed reaction/ biological activity

• H⁺ + orotidine 5'-phosphate --> CO₂ + UMP (according to UniProt)

Protein family

• OMP decarboxylase family (single member, according to UniProt)

Structure

• [PDB|1DBT]
• [AF|P25971]

Biological materials

Mutant

• BKE15550 ([gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAGTGTTTCTTCAGCTGACG,  downstream forward: _UP4_GCCTATAAGGCTGTCAGACT
• BKK15550 ([gene|5D0D0D2413377FCA92440334E92B5A27A5963D9E|pyrF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAGTGTTTCTTCAGCTGACG,  downstream forward: _UP4_GCCTATAAGGCTGTCAGACT

References

• 8206849,1709162,25326311,28189581