pyrE

168

orotate phosphoribosyltransferase

locus: BSU_15560

Molecular weight: 23.38 kDa

pI: 5.63

Protein length: 216 aa

Gene length: 651 bp

function: pyrimidine biosynthesis

product: orotate phosphoribosyltransferase

essential: no

ec: 2.4.2.10

synonyms: pyrE, pyrX

List of homologs in different organisms, belongs to COG0461 (Galperin et al., 2021)

Gene

Coordinates: 1,629,320  1,629,970

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The protein

Catalyzed reaction/ biological activity

• diphosphate + orotidine 5'-phosphate --> 5-phospho-α-D-ribose 1-diphosphate + orotate (according to UniProt)

Protein family

• [wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)

Structure

• [PDB|3DEZ] (from ''Streptococcus mutans'', 59% identity, 74% similarity)
• [AF|P25972]

Biological materials

Mutant

• BKE15560 ([gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAGATGTTTTGCGATGATTT,  downstream forward: _UP4_TAAAAAATAAATTCAAATGA
• BKK15560 ([gene|0197A037D2AF049B295E1DA60187D4614B2BAC51|pyrE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAGATGTTTTGCGATGATTT,  downstream forward: _UP4_TAAAAAATAAATTCAAATGA

References

• 8206849,1709162