Difference between revisions of "PyrD"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15540 pyrD]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15540 pyrD]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/pyrimidines_metabolism.html Pyrimidines], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pyrD pyrD]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 4.942   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 4.942   

Revision as of 10:44, 7 January 2014

  • Description: dihydroorotic acid dehydrogenase (catalytic subunit)

Gene name pyrD
Synonyms pyrDI
Essential no
Product dihydroorotic acid dehydrogenase (catalytic subunit)
Function pyrimidine biosynthesis
Gene expression levels in SubtiExpress: pyrD
Metabolic function and regulation of this protein in SubtiPathways:
pyrD
MW, pI 32 kDa, 4.942
Gene length, protein length 933 bp, 311 aa
Immediate neighbours pyrK, pyrF
Sequences Protein DNA DNA_with_flanks
Genetic context
PyrD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PyrD expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

PyrR regulon

The gene

Basic information

  • Locus tag: BSU15540

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-dihydroorotate + O2 = orotate + H2O2 (according to Swiss-Prot)
  • Protein family: Type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the absence of uridine nucleotides (PyrR) PubMed
  • Regulatory mechanism:
    • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References