Difference between revisions of "PyrC"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15500 pyrC]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15500 pyrC]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/pyrimidines_metabolism.html Pyrimidines], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=pyrC pyrC]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 5.427   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46 kDa, 5.427   

Revision as of 10:44, 7 January 2014

  • Description: dihydroorotase

Gene name pyrC
Synonyms
Essential no
Product dihydroorotase
Function pyrimidine biosynthesis
Gene expression levels in SubtiExpress: pyrC
Metabolic function and regulation of this protein in SubtiPathways:
pyrC
MW, pI 46 kDa, 5.427
Gene length, protein length 1284 bp, 428 aa
Immediate neighbours pyrB, pyrAA
Sequences Protein DNA DNA_with_flanks
Genetic context
PyrC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PyrC expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

PyrR regulon

The gene

Basic information

  • Locus tag: BSU15500

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate (according to Swiss-Prot)
  • Protein family: Type 2 subfamily (according to Swiss-Prot)
  • Paralogous protein(s): PucH

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3GRI (from Staphylococcus aureus subsp. aureus mw2, 60% identity, 72% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the absence of uridine nucleotides (PyrR) PubMed
  • Regulatory mechanism:
    • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yu-He Liang, Xiangyu Liu, Juan Wang, Lanfen Li, Xiao-Dong Su
Protein preparation, crystallization and preliminary X-ray crystallographic studies of dihydroorotase from Bacillus subtilis.
Protein Pept Lett: 2005, 12(7);717-9
[PubMed:16522191] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)