pyrC

168

dihydroorotase

locus: BSU_15500

Molecular weight: 46.45 kDa

pI: 5.43

Protein length: 428 aa

Gene length: 1287 bp

function: pyrimidine biosynthesis

product: dihydroorotase

essential: no

ec: 3.5.2.3

synonyms: pyrC

List of homologs in different organisms, belongs to COG0044 (Galperin et al., 2021)

Gene

Coordinates: 1,621,374 → 1,622,660

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The protein

Catalyzed reaction/ biological activity

• (S)-dihydroorotate + H₂O --> H⁺ + N-carbamoyl-L-aspartate (according to UniProt)

Protein family

• [wiki|Metallo-dependent hydrolases superfamily] (according to UniProt)

Structure

• [PDB|3GRI] (from ''Staphylococcus aureus subsp. aureus mw2'', 60% identity, 72% similarity)
• [AF|P25995]

Paralogous protein(s)

• [protein|8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A|pucH]

Biological materials

Mutant

• BKE15500 (Δ[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15500 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTTTCGTTTAGTATCCAGC,  downstream forward: _UP4_TATGAAGAGGGGAGACTTGT
• BKK15500 (Δ[gene|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15500 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATTTTCGTTTAGTATCCAGC,  downstream forward: _UP4_TATGAAGAGGGGAGACTTGT

References

• 8206849,1709162,16522191