Difference between revisions of "PyrB"

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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU15490
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P05654 P05654]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P05654 P05654]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15490 BSU15490]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15490]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.1.3.2 2.1.3.2]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.1.3.2 2.1.3.2]

Revision as of 12:00, 3 June 2009

  • Description: aspartate carbamoyltransferase

Gene name pyrB
Synonyms
Essential no
Product aspartate carbamoyltransferase
Function pyrimidine biosynthesis
MW, pI 34 kDa, 5.341
Gene length, protein length 912 bp, 304 aa
Immediate neighbours pyrP, pyrC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PyrB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU15490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate (according to Swiss-Prot)
  • Protein family: ATCase/OTCase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-303 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
  2. Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed