Difference between revisions of "PyrAB"

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<pubmed>8206849,1709162,,18763711, </pubmed>
 
<pubmed>8206849,1709162,,18763711, </pubmed>
# Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing ''Bacillus subtilis'' by divergent approaches. Proteomics '''8:''' 4123-4136 [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Revision as of 14:07, 15 June 2009

  • Description: carbamoyl-phosphate synthetase (catalytic subunit)

Gene name pyrAB
Synonyms
Essential no
Product carbamoyl-phosphate synthetase (catalytic subunit)
Function pyrimidine biosynthesis
MW, pI 117 kDa, 4.785
Gene length, protein length 3213 bp, 1071 aa
Immediate neighbours pyrAA, pyrK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PyrAB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU15520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)
  • Protein family: carB family (according to Swiss-Prot)
  • Paralogous protein(s): CarB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: membrane PubMed

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the absence of uridine nucleotides (PyrR) PubMed
  • Regulatory mechanism:
    • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References