Pyk

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  • Description: catalyzes the transfere of a phosphate from phosphoennolpyruvate to ADT and synthesis of pyruvate

Gene name pyk
Synonyms pykA
Essential no
Product Pyruvate kinase
Function kinase, transferase
MW, pI 61,9 kDa, 4.88
Gene length, protein length 1755 bp, 585 amino acids
Immediate neighbours pfkA, ytzA
Gene sequence (+200bp) Protein sequence
Genetic context
Pyk context.gif












The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + pyruvate = ADP + phosphoenolpyruvate
  • Protein family: pyruvate kinase family, (C-terminal section: PEP-utilizing enzyme family)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation
  • Cofactor(s): magnesium ion, potassium
  • Effectors of protein activity:
  • Interactions: Pyk-PfkA
  • Localization: cytoplasm PubMed

Database entries

  • Structure:
  • Swiss prot entry: [3]
  • KEGG entry: [4]
  • E.C. number: [5]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: twofold induced by glucose
  • Regulatory mechanism:
  • Additional information:

Biological materials

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References

  1. Ludwig, H., Homuth, G., Schmalisch, M., Dyka, F. M., Hecker, M., and Stülke, J. (2001) Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon. Mol Microbiol 41, 409-422.PubMed