PutR

• Description: transcriptional activator of the proline utilization operon putB-putC-putP
  
  

Gene name	putR
Synonyms	ycgP, prcR
Essential	no
Product	transcriptional activator (PucR family)
Function	regulation of proline utilization
Gene expression levels in SubtiExpress: putR
MW, pI	47 kDa, 5.37
Gene length, protein length	1233 bp, 411 aa
Immediate neighbours	putP, ycgQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, utilization of amino acids

The PutR regulon:

• putB-putC-putP

The gene

Basic information

• Locus tag: BSU03230

Phenotypes of a mutant

• no growth with proline as single source of carbon or nitrogen PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription activation of the proline utilization operon putB-putC-putP in the presence of proline PubMed

• Protein family: PucR family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): proline acts as co-activator PubMed

• Effectors of protein activity: proline activates PutR PubMed

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P94393

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: putR PubMed

• Expression browser: putR PubMed

• Sigma factor: SigA PubMed

• Regulation: constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Susanne Moses, Tatjana Sinner, Adrienne Zaprasis, Nadine Stöveken, Tamara Hoffmann, Boris R Belitsky, Abraham L Sonenshein, Erhard Bremer
Proline utilization by Bacillus subtilis: uptake and catabolism.
J Bacteriol: 2012, 194(4);745-58
[PubMed:22139509] [WorldCat.org] [DOI] (I p)

Shih-Chien Huang, Ta-Hui Lin, Gwo-Chyuan Shaw
PrcR, a PucR-type transcriptional activator, is essential for proline utilization and mediates proline-responsive expression of the proline utilization operon putBCP in Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 12);3370-3377
[PubMed:21964733] [WorldCat.org] [DOI] (I p)

Boris R Belitsky
Indirect repression by Bacillus subtilis CodY via displacement of the activator of the proline utilization operon.
J Mol Biol: 2011, 413(2);321-36
[PubMed:21840319] [WorldCat.org] [DOI] (I p)