putC

168

1-pyrroline-5-carboxylate dehydrogenase

locus: BSU_03210

Molecular weight: 56.32 kDa

pI: 5.37

Protein length: 515 aa

Gene length: 1548 bp

function: proline utilization

product: 1-pyrroline-5-carboxylate dehydrogenase

essential: no

ec: 1.5.1.12

synonyms: putC, ycgN

List of homologs in different organisms, belongs to COG1012 (Galperin et al., 2021)

Gene

Coordinates: 345,479  347,026

Phenotypes of a mutant

• no growth with proline as single source of carbon [Pubmed|21840319]
• the [gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC] [gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA] double mutant is unable to grow with proline, arginine, citrulline, or ornithine as the single source of nitrogen [pubmed|37343703]

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The protein

Catalyzed reaction/ biological activity

• H₂O + L-glutamate 5-semialdehyde + NAD⁺ --> 2 H⁺ + L-glutamate + NADH (according to UniProt)

Protein family

• [wiki|aldehyde dehydrogenase family] (according to UniProt)

Structure

• [PDB|3RJL] (1-pyrroline-5-carboxylate dehydrogenase from ''Bacillus licheniformis'', 90% identity, 97% similarity)
• [AF|P94391]

Paralogous protein(s)

• [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|dhaS], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA], [protein|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA], [protein|99F1FAF28FB4817D94E84BD5288FA33124558933|ywdH], [protein|A0BEB92D54799956A4ADE106A1388E5710141069|gabD], [protein|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT], [protein|F3341F205CB939498109D2A54DE842065C488DD5|aldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|aldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA]

Biological materials

Mutant

• GP3904 (''[gene|1F548515402950572E7212901729B2480432D1B9|putB]-[gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]''::''aphA3''), available in [wiki|Jörg Stülke]'s lab
• GP3749 ([gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]::aphA3 trpC2) available in [wiki|Jörg Stülke]'s lab [pubmed|37343703]
• GP3398 ([gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]::tet [gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]::aphA3 trpC2) available in [wiki|Jörg Stülke]'s lab [pubmed|37343703]
• MGNA-B993 (ycgN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1992 NBRP B. subtilis, Japan]
• BKE03210 ([gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTGTCATGATAATCTCTC,  downstream forward: _UP4_TAAGCGGGACTAAATGGGCA
• BKK03210 ([gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTGTCATGATAATCTCTC,  downstream forward: _UP4_TAAGCGGGACTAAATGGGCA

Expression vectors

• pGP3866: expression of Strep-''putC'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

References

• 12618455,14976255,14651647,21840319,23033921,21964733,22139509,36815589,37343703