Difference between revisions of "PurT"

From SubtiWiki
Jump to: navigation, search
m (Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk)
Line 100: Line 100:
 
* '''Operon:''' ''purT'' (according to [http://dbtbs.hgc.jp/COG/prom/purT.html DBTBS])
 
* '''Operon:''' ''purT'' (according to [http://dbtbs.hgc.jp/COG/prom/purT.html DBTBS])
  
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7496533}}
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=purT_243892_245046_1 purT] {{PubMed|22383849}}
 +
 
 +
* '''Sigma factor:''' [[SigA]] {{PubMed|7496533}}
 
   
 
   
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 106: Line 108:
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 15:05, 7 August 2012

  • Description: phosphoribosylglycinamide formyltransferase 2

Gene name purT
Synonyms
Essential no
Product phosphoribosylglycinamide formyltransferase 2
Function purine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Purine synthesis, Nucleotides (regulation)
MW, pI 41 kDa, 4.722
Gene length, protein length 1152 bp, 384 aa
Immediate neighbours ybfI, mpr
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PurT context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU02230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate (according to Swiss-Prot)
  • Protein family: ATP-grasp domain (according to Swiss-Prot)
  • Paralogous protein(s): PurK

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1KJ9 (with Mg-ATP from Escherichia coli, 55% identity, 69% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: purT (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

H H Saxild, J H Jacobsen, P Nygaard
Functional analysis of the Bacillus subtilis purT gene encoding formate-dependent glycinamide ribonucleotide transformylase.
Microbiology (Reading): 1995, 141 ( Pt 9);2211-8
[PubMed:7496533] [WorldCat.org] [DOI] (P p)