Difference between revisions of "PurS"

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|style="background:#ABCDEF;" align="center"|'''Function''' || purine biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || purine biosynthesis
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU06460 purS]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06460 purS]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PurS PurS]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PurS PurS]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[purC]]'', ''[[purQ]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[purC]]'', ''[[purQ]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12466&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06460 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:purS_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:purS_context.gif]]

Revision as of 12:17, 13 May 2013

  • Description: phosphoribosylformylglycinamidine synthase

Gene name purS
Synonyms yexA
Essential no
Product phosphoribosylformylglycinamidine synthase
Function purine biosynthesis
Gene expression levels in SubtiExpress: purS
Interactions involving this protein in SubtInteract: PurS
Metabolic function and regulation of this protein in SubtiPathways:
Purine synthesis, Nucleotides (regulation), Stress
MW, pI 9 kDa, 4.594
Gene length, protein length 252 bp, 84 aa
Immediate neighbours purC, purQ
Sequences Protein DNA Advanced_DNA
Genetic context
PurS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PurS expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

  • Locus tag: BSU06460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0062 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • repressed in the presence of adenine or adenosine(PurR) PubMed
    • repressed in the presence of guanine(G-box) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ruchi Anand, Aaron A Hoskins, Eric M Bennett, Michael D Sintchak, JoAnne Stubbe, Steven E Ealick
A model for the Bacillus subtilis formylglycinamide ribonucleotide amidotransferase multiprotein complex.
Biochemistry: 2004, 43(32);10343-52
[PubMed:15301532] [WorldCat.org] [DOI] (P p)

Aaron A Hoskins, Ruchi Anand, Steven E Ealick, JoAnne Stubbe
The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation.
Biochemistry: 2004, 43(32);10314-27
[PubMed:15301530] [WorldCat.org] [DOI] (P p)

Hans H Saxild, Per Nygaard
The yexA gene product is required for phosphoribosylformylglycinamidine synthetase activity in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 4);807-814
[PubMed:10784038] [WorldCat.org] [DOI] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis.
J Biol Chem: 1987, 262(17);8274-87
[PubMed:3036807] [WorldCat.org] (P p)