purS

168

phosphoribosylformylglycinamidine synthase

locus: BSU_06460

Molecular weight: 9.62 kDa

pI: 4.59

Protein length: 84 aa

Gene length: 255 bp

function: purine biosynthesis

product: phosphoribosylformylglycinamidine synthase

essential: no

ec: 6.3.5.3

synonyms: purS, yexA

List of homologs in different organisms, belongs to COG1828 (Galperin et al., 2021)

Gene

Coordinates: 702,319 702,573

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The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-glutamine + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide --> 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + ADP + H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• UPF0062 family (single member, according to UniProt)

Structure

• [PDB|1T4A]
• [AF|P12049]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• MGNA-A920 (yexA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/920 NBRP B. subtilis, Japan]
• BKE06460 ([gene|CCD4DA969086181CFA33D6D241F2CAAE02E0C758|purS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC
• BKK06460 ([gene|CCD4DA969086181CFA33D6D241F2CAAE02E0C758|purS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTAAGCTGACATAAACTT, downstream forward: _UP4_GAGGTTGAGGAGGTAGTCGC

References

• 15301532,15301530,10784038,3036807,7638212,15378759