PurR

• Description: transcription repressor of the pur operon
  
  

Gene name	purR
Synonyms	yabI
Essential	no
Product	transcription repressor of the pur operon
Function	regulation of purine biosynthesis
Gene expression levels in SubtiExpress: purR
Interactions involving this protein in SubtInteract: PurR
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation), Ala, Gly, Ser
MW, pI	31 kDa, 5.771
Gene length, protein length	855 bp, 285 aa
Immediate neighbours	ispE, yabJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, transcription factors and their control

This gene is a member of the following regulons

PurR regulon

The gene

Basic information

• Locus tag: BSU00470

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Biological activity: transcriptional repressor of purine biosynthetic operons

• Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed

• Interactions:
  • PurR-Prs

• Localization:

Database entries

• Structure: 1O57, 1O57 (complex with PRPP) NCBI PubMed

• UniProt: P37551

• KEGG entry: [3]

• E.C. number:

Additional information

requires YabJ for in vivo activity

Expression and regulation

• Operon: purR-yabJ PubMed

• Expression browser: purR PubMed

• Sigma factor:

• Regulation: negative autoregulation, induction in the presence of PRPP PubMed
  • repressed in the presence of purine nucleotides (PurR) PubMed

• Regulatory mechanism: repression by PurR PubMed
  • PurR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: