Difference between revisions of "PurR"

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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of purine biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of purine biosynthesis
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/ala_gly_ser.html Ala, Gly, Ser]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/pathways/ala_gly_ser.html Ala, Gly, Ser]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.771   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 5.771   

Revision as of 11:33, 18 June 2009

  • Description: transcription repressor of the pur operon

Gene name purR
Synonyms yabI
Essential no
Product transcription repressor of the pur operon
Function regulation of purine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation), Ala, Gly, Ser
MW, pI 31 kDa, 5.771
Gene length, protein length 855 bp, 285 aa
Immediate neighbours ispE, yabJ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PurR context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU00470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Biological activity: transcriptional repressor of purine biosynthetic operons
  • Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

requires YabJ for in vivo activity

Expression and regulation

  • Regulation: negative autoregulation, induction in the presence of PRPP PubMed
    • repressed in the presence of purine nucleotides (PurR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References