PurR

• Description: purine repressor
  
  

Gene name	purR
Synonyms	yabI
Essential	no
Product	transcriptional regulator
Function	negative regulation of the purine operons ( purEKBCLQFMNHD, purA)
MW, pI	31 kDa, 5.771
Gene length, protein length	855 bp, 285 aa
Immediate neighbours	ispE, yabJ
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Biological activity: transcriptional repressor of purine biosynthetic operons

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed

• Interactions:

• Localization:

Database entries

• Structure: 1P4A (complex with PRPP) NCBI PubMed

• Swiss prot entry:

• KEGG entry:

• E.C. number:

Additional information

requires YabJ for in vivo activity

Expression and regulation

• Operon: purR-yabJ PubMed

• Sigma factor:

• Regulation: negative autoregulation PubMed

• Regulatory mechanism: repression by PurR PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Weng et al. (1995) Identification of the Bacillus subtilis pur operon repressor. Proc. Natl. Acad. Sci. USA 92: 7455-7459. PubMed
2. Weng & Zalkin (2000) Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.Currr. Microbiol. 41: 56-59. PubMed
3. Sinha et al. (2003) The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation.J. Bacteriol. 185: 4087-4098. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed