PurN

From SubtiWiki
Revision as of 10:17, 7 January 2014 by 134.76.70.252 (talk)
Jump to: navigation, search
  • Description: phosphoribosylglycinamide formyltransferase, irreversible

Gene name purN
Synonyms
Essential no
Product phosphoribosylglycinamide formyltransferase
Function purine biosynthesis
Gene expression levels in SubtiExpress: purN
Metabolic function and regulation of this protein in SubtiPathways:
purN
MW, pI 21 kDa, 6.182
Gene length, protein length 585 bp, 195 aa
Immediate neighbours purM, purH
Sequences Protein DNA DNA_with_flanks
Genetic context
PurN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PurN expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

  • Locus tag: BSU06510

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide (according to Swiss-Prot)
  • Protein family: GART family (according to Swiss-Prot)
  • Paralogous protein(s): YkkE

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3P9X (from B. halodurans, 51% identity, 70% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • repressed in the presence of adenine or adenosine(PurR) PubMed
    • repressed in the presence of guanine(G-box) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yu-He Liang, Xiang-Yu Liu, Juan Wang, Lan-Fen Li
Protein preparation, crystallization and preliminary crystallographic studies of Bacillus subtilis glycinamide ribonucleotide transformylase.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 7);709-11
[PubMed:19574646] [WorldCat.org] [DOI] (I p)

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc Natl Acad Sci U S A: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)

H H Saxild, J H Jacobsen, P Nygaard
Genetic and physiological characterization of a formate-dependent 5'-phosphoribosyl-1-glycinamide transformylase activity in Bacillus subtilis.
Mol Gen Genet: 1994, 242(4);415-20
[PubMed:8121396] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis.
J Biol Chem: 1987, 262(17);8274-87
[PubMed:3036807] [WorldCat.org] (P p)