Difference between revisions of "PurL"

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|style="background:#ABCDEF;" align="center"|'''Function''' || purine biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || purine biosynthesis
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU06480 purL]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU06480 purL]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PurL PurL]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PurL PurL]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[purQ]]'', ''[[purF]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[purQ]]'', ''[[purF]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12468&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06480 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06480 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06480 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:purL_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:purL_context.gif]]

Revision as of 12:17, 13 May 2013

  • Description: phosphoribosylformylglycinamidine synthase

Gene name purL
Synonyms
Essential no
Product phosphoribosylformylglycinamidine synthase
Function purine biosynthesis
Gene expression levels in SubtiExpress: purL
Interactions involving this protein in SubtInteract: PurL
Metabolic function and regulation of this protein in SubtiPathways:
Purine synthesis, Nucleotides (regulation)
MW, pI 80 kDa, 4.554
Gene length, protein length 2226 bp, 742 aa
Immediate neighbours purQ, purF
Sequences Protein DNA Advanced_DNA
Genetic context
PurL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PurL expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, membrane proteins

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

  • Locus tag: BSU06480

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate (according to Swiss-Prot)
  • Protein family: FGAMS family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3D54 (from Thermotoga maritima, 39% identity, 58% similarity) PubMed
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • repressed in the presence of adenine or adenosine(PurR) PubMed
    • repressed in the presence of guanine(G-box) PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed