purK

168

phosphoribosylaminoimidazole carboxylase (ATP-dependent)

locus: BSU_06430

Molecular weight: 42.00 kDa

pI: 5.12

Protein length: 380 aa

Gene length: 1143 bp

function: purine biosynthesis

product: phosphoribosylaminoimidazole carboxylase (ATP-dependent)

essential: no

ec: 4.1.1.21

synonyms: purK

List of homologs in different organisms, belongs to COG0026 (Galperin et al., 2021)

Gene

Coordinates: 699,093  700,235

Advertisement

The protein

Catalyzed reaction/ biological activity

• 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + ATP + hydrogencarbonate --> 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H⁺ + phosphate(according to UniProt)

Protein family

• PurK/PurT family (with [protein|C00E34EF63B5EAE33CAD5E469893C0A5112B9F35|purT], according to UniProt)

[wiki|Domains]

• [wiki|ATP-grasp doamin] (aa 112-298) (according to UniProt)

Structure

• [PDB|2Z04] (from ''Aquifex aeolicus'', 37% identity, 57% similarity)
• [AF|P12045]

Paralogous protein(s)

• [protein|C00E34EF63B5EAE33CAD5E469893C0A5112B9F35|purT]

Biological materials

Mutant

• BKE06430 ([gene|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|purK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TACAGCTCCCGGATAGATGA,  downstream forward: _UP4_AATAGAGACGGAGGACAAGC
• BKK06430 ([gene|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|purK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TACAGCTCCCGGATAGATGA,  downstream forward: _UP4_AATAGAGACGGAGGACAAGC

References

• 3036807,12923093,7638212