purH

168

phosphoribosylaminoimidazole carboxamide formyltransferase

locus: BSU_06520

Molecular weight: 55.57 kDa

pI: 5.13

Protein length: 512 aa

Gene length: 1539 bp

function: purine biosynthesis

product: phosphoribosylaminoimidazole carboxamide formyltransferaseand inosine-monophosphate cyclohydrolase

essential: no

ec: 2.1.2.3

synonyms: purH, purJ

List of homologs in different organisms, belongs to COG0138 (Galperin et al., 2021)

Gene

Coordinates: 708,594 710,132

Phenotypes of a mutant

• more resistant than wild-type to zinc limitation when grown on rich medium (LB), this is blocked by simultaneous deletion of [gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB] (reason: accumulation of ZTP that stimulates the binding of [protein|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA] to [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE], and thus folate biosynthesis during zinc limitation) [pubmed|31132310]

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The protein

Catalyzed reaction/ biological activity

• (6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamide --> (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)
• H₂O + IMP --> 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)

Protein family

• purH family (single member, according to UniProt)

[wiki|Domains]

• MGS-like domain (aa 1-146) (according to UniProt)

Structure

• [PDB|1ZCZ] (from ''Thermotoga maritima'', 36% identity, 54% similarity) [Pubmed|18260100]
• [AF|P12048]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• BKE06520 ([gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA
• BKK06520 ([gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATTAATGCACGTTTAA, downstream forward: _UP4_AAACATTAAGGGGATGAAAA

References

• 3036807,12923093,15378759,7638212,31132310