purF

purF
168

glutamine phosphoribosyldiphosphate amidotransferase

locus
BSU_06490
Molecular weight
51.45 kDa
pI
5.87
Protein length
Gene length
function
purine biosynthesis
product
glutamine phosphoribosyldiphosphate amidotransferase
essential
no
ec
2.4.2.14
synonyms
purF, purB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0034 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
705,441  706,871
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The protein
Catalyzed reaction/ biological activity
5-phospho-β-D-ribosylamine + diphosphate + L-glutamate --> 5-phospho-α-D-ribose 1-diphosphate + H2O + L-glutamine (according to UniProt)
Protein family
[wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-2 domain] (aa 12-231) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|1GPH],  [PDB|1AO0] (complex with ADP and GMP)
[AF|P00497]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Expression and Regulation
Operons
genes
[gene|1A9F7847AA1EB2A264842B44CD1B1A21F0AF6E57|purE]-[gene|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|purK]-[gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB]-[gene|447D12E940C7F45B6C5015844F9DED9D513CD6E5|purC]-[gene|CCD4DA969086181CFA33D6D241F2CAAE02E0C758|purS]-[gene|DEE856E83E790EB57F103F7A9156408AF090995D|purQ]-[gene|D34203FAA84C19B34B3CB5F00AC2E2663FFA344D|purL]-[gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]-[gene|AD0DE9457D70DF02FA0F58867B24ABF236FAF65A|purM]-[gene|7B30F27D6C459437967A64467E52961F4A9E0F2C|purN]-[gene|54CE8EB2F5D1F9AEAA497E1F60248C9B2250B01E|purH]-[gene|9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E|purD]
description
[Pubmed|3036807]
regulation
expression activated by glucose (4.4 fold) [Pubmed|12850135]
regulatory mechanism
G-box: RNA switch, ([wiki|riboswitch]) [pubmed|3036807,12787499], in [regulon|other_regulator:G-box|G-box]
[protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: repression, (molecular inducer: PRPP) [Pubmed|7638212,2536750], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|3036807], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|1A9F7847AA1EB2A264842B44CD1B1A21F0AF6E57|purE]→[gene|9BBA22B09C6DD6C36E043D44B0F54BD0FFA5BA0E|purD]

2024-03-18 19:57:57

Jstuelk

142

ace69dc80f4f087486c218727fa370a7a9630d21

EDD8EC37B0E5B38941D5827C265AB2F23CF23EA7

Biological materials
Mutant
BKE06490 ([gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT,  downstream forward: _UP4_TAAAACTTGAAAAATGACAT
BKK06490 ([gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT,  downstream forward: _UP4_TAAAACTTGAAAAATGACAT
References
Reviews
12859215,11395405
Original Publications
6315725,12923093,6794614,2553706,6401710,6771260,2495277,109433,6814966,6411716,6411717,6794613,3036807,8197456,7638212,25023436

4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952

Page visits: 3974

Time of last update: 2024-03-28 16:04:43

Author of last update: Melvin.boenninger