purF
168
glutamine phosphoribosyldiphosphate amidotransferase
locus
BSU_06490
Molecular weight
51.45 kDa
pI
5.87
function
purine biosynthesis
product
glutamine phosphoribosyldiphosphate amidotransferase
essential
no
ec
2.4.2.14
synonyms
purF, purB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0034 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
705,441 706,871
The protein
Catalyzed reaction/ biological activity
5-phospho-β-D-ribosylamine + diphosphate + L-glutamate --> 5-phospho-α-D-ribose 1-diphosphate + H2O + L-glutamine (according to UniProt)
Protein family
[wiki|Purine/pyrimidine phosphoribosyltransferase family] (according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-2 domain] (aa 12-231) (according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|1GPH], [PDB|1AO0] (complex with ADP and GMP)
[AF|P00497]
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
Biological materials
Mutant
BKE06490 ([gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT, downstream forward: _UP4_TAAAACTTGAAAAATGACAT
BKK06490 ([gene|4AA5AF9C7CE8D0A9FD3A47650ECF130BCBEE1952|purF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT, downstream forward: _UP4_TAAAACTTGAAAAATGACAT
References
Reviews
Original Publications
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Time of last update: 2024-04-24 12:09:47
Author of last update: Melvin.boenninger