purE

168

phosphoribosylaminoimidazole carboxylase (ATP-dependent)

locus: BSU_06420

Molecular weight: 17.03 kDa

pI: 5.2

Protein length: 162 aa

Gene length: 489 bp

function: purine biosynthesis

product: phosphoribosylaminoimidazole carboxylase (ATP-dependent)

essential: no

ec: 4.1.1.21

synonyms: purE

List of homologs in different organisms, belongs to COG0041 (Galperin et al., 2021)

Gene

Coordinates: 698,612  699,100

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The protein

Catalyzed reaction/ biological activity

• 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H⁺ --> 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate (according to UniProt)

Protein family

• AIR carboxylase family ( single member, according to UniProt)

Structure

• [PDB|1O4V] (from ''Thermotoga maritima'', 53% identity, 64% similarity) [Pubmed|15048837]
• [AF|P12044]

Biological materials

Mutant

• BKE06420 ([gene|1A9F7847AA1EB2A264842B44CD1B1A21F0AF6E57|purE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTGTTCCCCACCTTCT,  downstream forward: _UP4_ACAGTGTTAGAAAGCAGTGA
• BKK06420 ([gene|1A9F7847AA1EB2A264842B44CD1B1A21F0AF6E57|purE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTGTTCCCCACCTTCT,  downstream forward: _UP4_ACAGTGTTAGAAAGCAGTGA

References

• 12787499,3036807,12923093,7638212,35622174