purC

168

phosphoribosylaminoimidazole succinocarboxamide synthase

locus: BSU_06450

Molecular weight: 27.31 kDa

pI: 4.88

Protein length: 241 aa

Gene length: 726 bp

function: purine biosynthesis

product: phosphoribosylaminoimidazole succinocarboxamide synthase

essential: no

ec: 6.3.2.6

synonyms: purC

List of homologs in different organisms, belongs to COG0152 (Galperin et al., 2021)

Gene

Coordinates: 701,601 702,326

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The protein

Catalyzed reaction/ biological activity

• 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate --> (2S)-2-[5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H⁺ + phosphate (according to UniProt)

Protein family

• SAICAR synthetase family (according to Swiss-Prot)

Structure

• [PDB|1KUT] (from ''Thermotoga maritima'', 40% identity, 57% similarity) [Pubmed|16582479]
• [AF|P12046]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• BKE06450 ([gene|447D12E940C7F45B6C5015844F9DED9D513CD6E5|purC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGGAAGGCCTCCTAACCC, downstream forward: _UP4_TTCAATAGACTGGGAGGCAT
• BKK06450 ([gene|447D12E940C7F45B6C5015844F9DED9D513CD6E5|purC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGGAAGGCCTCCTAACCC, downstream forward: _UP4_TTCAATAGACTGGGAGGCAT

References

• 12884008,3036807,12923093,7638212,15378759