purB

168

adenylsuccinate lyase

locus: BSU_06440

Molecular weight: 49.32 kDa

pI: 5.82

Protein length: 431 aa

Gene length: 1296 bp

function: purine biosynthesis

product: adenylsuccinate lyase

essential: no

ec: 4.3.2.2

synonyms: purB

List of homologs in different organisms, belongs to COG0015 (Galperin et al., 2021)

Gene

Coordinates: 700,232 701,527

Phenotypes of a mutant

• poor growth [pubmed|28189581]
• non-transformable [pubmed|28189581]

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The protein

Catalyzed reaction/ biological activity

• N⁶-(1,2-dicarboxyethyl)-AMP --> AMP + fumarate (according to UniProt)
• (2S)-2-[5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamido]succinate --> 5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamide + fumarate (according to UniProt)

Protein family

• [wiki|lyase 1 family] (according to UniProt)

Structure

• [PDB|1F1O]
• [AF|P12047]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• BKE06440 ([gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC
• BKK06440 ([gene|29FF54E2B9F4A11811F5E15885A4C02A259C7B35|purB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC

References

• 1722815,3036807,12923093,15378759,7638212,28189581