Difference between revisions of "PurA"

From SubtiWiki
Jump to: navigation, search
Line 37: Line 37:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
+
<br/><br/>
<br/><br/><br/><br/>
 
 
 
 
 
<br/><br/><br/><br/><br/><br/>
 
  
 
= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}},
 
{{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}},
{{SubtiWiki category|[[phosphoproteins]]}}
+
{{SubtiWiki category|[[phosphoproteins]]}},
 +
[[most abundant proteins]]
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
Line 65: Line 62:
  
 
=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
Line 83: Line 77:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
 
** phosphorylated on Arg-97 {{PubMed|22517742}}
 
** phosphorylated on Arg-97 {{PubMed|22517742}}
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
Line 115: Line 109:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=purA_4155433_4156725_-1 purA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=purA_4155433_4156725_-1 purA] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|1312531}}  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|1312531}}  
  
 
* '''Regulation:''' expression activated by glucose (4.9 fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]   
 
* '''Regulation:''' expression activated by glucose (4.9 fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]   
Line 124: Line 118:
  
 
* '''Additional information:'''
 
* '''Additional information:'''
 +
** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}}
  
 
=Biological materials =
 
=Biological materials =
Line 145: Line 140:
 
=References=
 
=References=
  
<pubmed>1722815,1312531 ,7638212,12850135, 21424839 22517742</pubmed>
+
<pubmed>1722815,1312531 ,7638212,12850135, 21424839 22517742 15378759</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:56, 5 March 2014

  • Description: adenylosuccinate synthetase

Gene name purA
Synonyms
Essential no
Product adenylosuccinate synthetase
Function purine biosynthesis
Gene expression levels in SubtiExpress: purA
Metabolic function and regulation of this protein in SubtiPathways:
purA
MW, pI 47 kDa, 5.459
Gene length, protein length 1290 bp, 430 aa
Immediate neighbours trnY-Lys, yycE
Sequences Protein DNA DNA_with_flanks
Genetic context
PurA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PurA expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, phosphoproteins, most abundant proteins

This gene is a member of the following regulons

PurR regulon

The gene

Basic information

  • Locus tag: BSU40420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP (according to Swiss-Prot)
  • Protein family: adenylosuccinate synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated on Arg-97 PubMed
  • Effectors of protein activity:

Database entries

  • Structure: 1KJX (from Escherichia coli k12, 46% identity, 64% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: expression activated by glucose (4.9 fold) (CcpA) PubMed
    • repressed in the presence of purine nucleotides (PurR) PubMed
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References