purA

168

adenylosuccinate synthetase

locus: BSU_40420

Molecular weight: 47.51 kDa

pI: 5.46

Protein length: 430 aa

Gene length: 1293 bp

function: purine biosynthesis

product: adenylosuccinate synthetase

essential: no

ec: 6.3.4.4

synonyms: purA

List of homologs in different organisms, belongs to COG0104 (Galperin et al., 2021)

Gene

Coordinates: 4,155,433 4,156,725

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The protein

Catalyzed reaction/ biological activity

• GTP + IMP + L-aspartate --> GDP + 2 H⁺ + N⁶-(1,2-dicarboxyethyl)-AMP + phosphate (according to UniProt)

Protein family

• adenylosuccinate synthetase family (single member, according to UniProt)

Structure

• [PDB|1KJX] (from ''Escherichia coli k12'', 46% identity, 64% similarity) [Pubmed|11741996]
• [AF|P29726]

Modification

• phosphorylated on Arg-97 [Pubmed|22517742]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• BKE40420 ([gene|AB4B8B7325A1BF444D841EF1BBBCEC2D506AE600|purA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCGTGCACCTCCGTT, downstream forward: _UP4_TAAATAGAATATGTCTGCAA
• BKK40420 ([gene|AB4B8B7325A1BF444D841EF1BBBCEC2D506AE600|purA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCCGTGCACCTCCGTT, downstream forward: _UP4_TAAATAGAATATGTCTGCAA

References

• 1722815,1312531,7638212,12850135,21424839,22517742,15378759,27234879,30974118