PucM

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  • Description: uricase

Gene name pucM
Synonyms yunM
Essential no
Product uricase
Function purine utilization
Gene expression levels in SubtiExpress: pucM
Metabolic function and regulation of this protein in SubtiPathways:
pucM
MW, pI 13 kDa, 5.082
Gene length, protein length 363 bp, 121 aa
Immediate neighbours pucL, yuzJ
Sequences Protein DNA DNA_with_flanks
Genetic context
PucM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PucM expression.png




























Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU32460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate (according to Swiss-Prot)
  • Protein family: 5-hydroxyisourate hydrolase subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References