pucM

168

uricase

locus: BSU_32460

Molecular weight: 13.27 kDa

pI: 5.08

Protein length: 114 aa

Gene length: 345 bp

function: purine utilization

product: uricase

essential: no

ec: 3.5.2.17

synonyms: pucM, yunM

List of homologs in different organisms, belongs to COG2351 (Galperin et al., 2021)

Gene

Coordinates: 3,334,646  3,334,990

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The protein

Catalyzed reaction/ biological activity

• 5-hydroxyisourate + H₂O --> 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H⁺ (according to UniProt)

Protein family

• transthyretin family (according to UniProt)

Structure

• [PDB|2H0E] [Pubmed|16782815]
• [AF|O32142]

Biological materials

Mutant

• MGNA-A938 (yunM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/938 NBRP B. subtilis, Japan]
• BKE32460 ([gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATATGGGTTGTCAGTTTTC,  downstream forward: _UP4_TAAGAAGGAAGCCCGCCTCA
• BKK32460 ([gene|A2F93FFE225DFDBCBB3EA079BEF84C79465F0E5C|pucM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATATGGGTTGTCAGTTTTC,  downstream forward: _UP4_TAAGAAGGAAGCCCGCCTCA

References

• 11344136,12823818,12029039,16782815,20168977,25755103