pucL

168

uricase, urate oxidase

locus: BSU_32450

Molecular weight: 56.39 kDa

pI: 5.48

Protein length: 494 aa

Gene length: 1485 bp

function: purine utilization

product: uricase

essential: no

ec: 1.7.3.3

synonyms: pucL, yunL

List of homologs in different organisms, belongs to COG3648 (Galperin et al., 2021)

Gene

Coordinates: 3,333,162  3,334,646

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The protein

Catalyzed reaction/ biological activity

• 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H⁺ --> (S)-allantoin + CO₂ (according to UniProt)
• H₂O + O₂ + urate --> 5-hydroxyisourate + H₂O₂ (according to UniProt)

Protein family

• N-terminal part: OHCU decarboxylase family (single member, according to UniProt)
• C-terminal part: uricase family (single member, according to UniProt)

Structure

• [PDB|6A4M]
• [AF|O32141]

Biological materials

Mutant

• MGNA-A937 (yunL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/937 NBRP B. subtilis, Japan]
• BKE32450 ([gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTGAACATTGGCATCT,  downstream forward: _UP4_AAATGTCGGAGCCTGAAAGC
• BKK32450 ([gene|2E7DEC53A130247E66225E87950D702E462C324F|pucL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTGAACATTGGCATCT,  downstream forward: _UP4_AAATGTCGGAGCCTGAAAGC

References

• 11344136,25755103,12823818,12029039,20168977,28531234