Difference between revisions of "PucL"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32450&redirect=T BSU32450]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pucRJKLM.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pucRJKLM.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU32450&redirect=T BSU32450]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1J2G 1J2G] (with 8-Azaxanthine from ''Bacillus sp.'', 66% identity, 79% similarity)
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1J2G 1J2G] (with 8-Azaxanthine from ''Bacillus sp.'', 66% identity, 79% similarity)

Revision as of 14:40, 2 April 2014

  • Description: uricase, urate oxidase

Gene name pucL
Synonyms yunL
Essential no
Product uricase
Function purine utilization
Gene expression levels in SubtiExpress: pucL
Metabolic function and regulation of this protein in SubtiPathways:
pucL
MW, pI 56 kDa, 5.484
Gene length, protein length 1482 bp, 494 aa
Immediate neighbours pucK, pucM
Sequences Protein DNA DNA_with_flanks
Genetic context
PucL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PucL expression.png















Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU32450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO2 (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1J2G (with 8-Azaxanthine from Bacillus sp., 66% identity, 79% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Pollyana Pfrimer, Lidia Maria Pepe de Moraes, Alexsandro Sobreira Galdino, Loise Pedrosa Salles, Viviane Castelo Branco Reis, Janice Lisboa De Marco, Maura Vianna Prates, Carlos Bloch, Fernando Araripe Gonçalves Torres
Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli.
J Biomed Biotechnol: 2010, 2010;674908
[PubMed:20168977] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)