Difference between revisions of "PucK"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
 
 
* '''Operon:'''  
 
* '''Operon:'''  
 +
** ''[[pucR]]-[[pucJ]]-[[pucK]]-[[pucL]]-[[pucM]]'' {{PubMed|12823818}}
 +
** ''[[pucJ]]-[[pucK]]-[[pucL]]-[[pucM]]'' {{PubMed|12029039}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
 +
** ''[[pucJ]]'': [[SigA]] {{PubMed|12029039}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12823818 PubMed]
+
** expressed in the absence of good nitrogen sources (glutamine or ammonium) ([[TnrA]]) {{PubMed|12823818}}
** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12029039 PubMed]
+
** induced in the presence of purine nucleotides (inducer: allantoin) ([[PucR]]) {{PubMed|12029039}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[TnrA]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12823818 PubMed]
+
** [[TnrA]]: transcription activation {{PubMed|12823818}}
** [PucR]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/12029039 PubMed]
+
** [[PucR]]: transcription activation {{PubMed|12029039}}
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 15:24, 28 December 2009

  • Description: uric acid permease

Gene name pucK
Synonyms yunK
Essential no
Product uric acid permease
Function purine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Nucleotides (regulation)
MW, pI 44 kDa, 8.348
Gene length, protein length 1290 bp, 430 aa
Immediate neighbours pucJ, pucL
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PucK context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU32440

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: xanthine/uracil permease family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)