Difference between revisions of "PucI"

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|style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_catabolism.html Purine catabolism], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 7.882   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 53 kDa, 7.882   

Revision as of 13:16, 18 June 2009

  • Description: allantoin permease

Gene name pucI
Synonyms ywoE
Essential no
Product allantoin permease
Function purine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Nucleotides (regulation)
MW, pI 53 kDa, 7.882
Gene length, protein length 1470 bp, 490 aa
Immediate neighbours ywoF, ywoD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwoE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU36470

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: purine-cytosine permease family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Regulatory mechanism:
    • [PucR]]: transcription activation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)