pucI

168

allantoin permease

locus: BSU_36470

Molecular weight: 53.82 kDa

pI: 7.88

Protein length: 490 aa

Gene length: 1473 bp

function: purine utilization

product: allantoin permease

essential: no

synonyms: pucI, ywoE

List of homologs in different organisms, belongs to COG1953 (Galperin et al., 2021)

Gene

Coordinates: 3,752,280  3,753,752

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The protein

Catalyzed reaction/ biological activity

• uptake of allantoin [Pubmed|26967546]

Protein family

• purine-cytosine permease (2.A.39) family (with [protein|CB969A2D60F106466605052299F2A5FF5A20E9ED|yxlA], according to UniProt)

Structure

• [PDB|2JLN] (from Microbacterium liquefaciens, 27% identity) [pubmed|18927357]
• [AF|P94575]

[wiki|Localization]

• cell membrane [Pubmed|26967546]

Biological materials

Mutant

• MGNA-A897 (ywoE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/897 NBRP B. subtilis, Japan]
• BKE36470 ([gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCCGGCTCCCCTTTCC,  downstream forward: _UP4_TAAGATCTATTTAGACGGTA
• BKK36470 ([gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCCGGCTCCCCTTTCC,  downstream forward: _UP4_TAAGATCTATTTAGACGGTA
• BP1261 ([gene|5681A5F186AD4B8C81B78FD9DDAE2C0624BE7CAF|pucI]::cat trp+) available in [wiki|Jörg Stülke]'s & [wiki|Fabian Commichau]'s lab

References

• 11344136,12029039,12029039,26967546,27766092,18927357